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Zamir Punja, PhD is a Professor of Plant Biotechnology at Simon Fraser University in Canada. His research interests include the etiology and management of plant diseases on vegetable and horticultural crops, and the applications of plant biotechnology for disease management. Since 2018, his work has shifted to researching cannabis, where his group has described a range of previously unreported pathogens affecting the crop and has evaluated various methods for disease management. At CannMed 25, Zamir will present his team’s latest work investigating the microbiome of cannabis and hemp plants. Using Next-Generation Sequencing and microscopy, his team identified diverse fungi, yeasts, and bacteria across plant tissues. Their study reveals that cannabis plants host abundant microbial endophytes, which can impact plant health and product quality. Findings also highlight the influence of growing substrates and biocontrol agents on the cannabis microbiome. During our conversation, we discuss: * What is the microbiome and how do microbial endophytes affect plant health * Identification of common microbes found in cannabis samples, including Penicillium and Fusarium. * Whether meristem tissue culture is a viable microbial sterilization method. * The influence of sterilized vs. non-sterilized growing media. * How growers can use microbiome analysis for early interventions. * Insights into beneficial fungi like Trichoderma and Mycorrhizae. * Future research questions on microbial balance and seed microbiome engineering. * And More Thanks to This Episode’s Sponsor: GrowDefender GrowDefender provides long-lasting antimicrobial protection for indoor cultivation and processing spaces. Using patented Goldshield® technology, their EPA-registered, water-based products protect surfaces from mold, mildew, bacteria, and fungi for up to 90 days. Safe, non-toxic, and easy to apply, GrowDefender helps growers reduce contamination, protect crops, and maintain cleaner, healthier environments. Learn more at growdefender.com Additional Resources - Endophytes in Cannabis sativa: Identifying and Characterizing Microbes with Beneficial and Detrimental Effects on Plant Health - https://pmc.ncbi.nlm.nih.gov/articles/PMC12030312/ - Exploring the fascinating development of cannabinoid-producing trichomes - https://youtu.be/Avhr8XjsBTU?si=nbYpjuoMMnKVqF6X - Register for CannMed 25 - https://cvent.me/emBPno - Meet the CannMed 25 Speakers and Poster Presenters - https://cannmedevents.com/speakers/ - Review the Podcast - https://podcasts.apple.com/us/podcast/cannmed- - CannMed Archive - coffee-talk/id1504218804 - https://cannmedevents.com/cannmed-video-archives/

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In the final episode of our How Hemp CBD Products Are Made video series, we learn how the hemp food company Manitoba Harvest overcame the obstacles involved in crafting a CBD-rich protein powder. To that make the most out of every seed, their team of food scientists employs a variety of techniques to blend hemp protein concentrate and broad-spectrum hemp extract with other nutritious ingredients. The result is a consistent serving of protein packed powder that makes it easy to add CBD, along with vitamins and minerals, to a post-workout drink, a breakfast smoothie, or anywhere else you want it in your diet. ------------------------- This product has intoxicating effects and may be habit forming. Marijuana can impair concentration, coordination, and judgment. Do not operate a vehicle or machinery under the influence of this drug. There may be health risks associated with consumption of this product. For use only by adults twenty-one and older. Keep out of the reach of children.

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